With increasing mental health challenges among university students, horticultural therapy— using nature and plant-based activities—has gained attention as a psychological intervention method. This study explores the application pathways of horticultural therapy within the “Five-in-One” mental health education system in Chinese universities. By examining how horticultural therapy integrates into education and teaching, practical activities, counseling services, prevention and intervention, and platform support, it proposes specific strategies for incorporating horticultural therapy into curricula, activities, and counseling. Additionally, the study emphasizes the importance of platform support, such as facilities, curriculum development, teacher training, and resource integration. This research provides new perspectives for university mental health education, facilitating the broader application of horticultural therapy and enhancing student well-being.