This study aims to explore the application and symbolic significance of tree imagery in Jungian analytical psychology. Through art therapy, the study analyzes how tree imagery serves as a bridge between consciousness and the unconscious, facilitating self-understanding and healing in individuals. The study hypothesizes a positive correlation between changes in tree imagery during art therapy sessions and improvements in mental well-being. That the use of tree imagery in art therapy will achieve selfgrowth and enhance inner strength. Adopting qualitative research design, data is collected through tree imagery paintings and interview records, focusing on the individual’s experience of using tree imagery in treatment. The study also considers the influence of cultural background on the understanding of symbolic meanings, particularly within the Chinese cultural context. The results indicate that tree imagery is effective in enhancing self-awareness, emotional healing, and personal growth, offering new perspectives and methods for Jungian therapeutic practice. Simultaneously, the study points out limitations in current practices and suggests future research directions. Empirical data for the field of Jungian psychology is provided. The research deepens the understanding of prototype theory, offers important insights for theoretical and practical applications in psychotherapy.