Objective: To investigate the relationship between Non-Suicidal Self-Injury (NSSI) behavior, emotional regulation strategies, and ineffective family environment among college students. Method: A random cluster sampling method was employed to select 1068 college students from ffve universities including Sichuan University of Industry and Technology, Ziyang Stomatological College, Chengdu University of Arts and Sciences, Xiangyang Automobile Vocational and Technical College, and Geely College for a questionnaire survey. The Non-Suicidal Self-Injury Behavior Questionnaire (ANSAQ), Emotion Regulation Questionnaire (ERQ), and China Ineffective Family Scale (CIFS) were utilized to assess non-suicidal self-injury behavior, emotional regulation strategies, and ineffective family environment among college students. The relationship between NSSI behavior, emotional regulation strategies, and ineffective family environment was analyzed using χ²-test, independent sample t-test, and logistic regression analysis. The overall NSSI detection rate among college students was 15.50%. College students with NSSI behavior exhibited signiffcantly higher scores in expression inhibition compared to those without NSSI. College students with NSSI behavior scored signiffcantly higher in father total score, father emotional imbalance, father overemphasizing grades, father neglecting care, father refusing, mother total score, mother emotional imbalance, mother overemphasizing grades, mother neglecting care, mother refusing, and mother psychological control than those without NSSI behavior. Logistic regression analysis results indicated that expression inhibition score, maternal negation of child experience, and maternal psychological control were risk factors for NSSI behavior in college students. Conclusion: The NSSI behavior of college students is significantly correlated with emotional regulation strategies and ineffective family environments. Attention should be given to the physical and mental health of college students to reduce the incidence of NSSI.