This research attempts to understand the effect of positive psychological quality training camp on the subjective well-being of higher vocational college students, and provide reference for the construction of the characteristic curriculum system of positive psychological practice in higher vocational colleges. This research adopted quasi-experimental design, and took 114 students in a higher vocational college as the research object, a 21-day positive psychological quality training camp with the theme of Gratitude Diary and Life Control was conducted in groups, the General Well-Being Schedule, Gratitude Questionnaire-6 and Sense of Time Control Schedule were used to investigate the differences between positive psychological quality and subjective well-being. The results showed that the post-test of gratitude level in Gratitude Diary group was significantly higher than that in the pre-test (t= 2.947, p<0.005) , and the post-test of Life Control group was significantly higher than that in the pre-test (t=2.738, p<0.05; t=2.072, p<0.05), and the post-test of subjective well-being in both groups were significantly higher than the pre-test (t=4.000, p< 0.001; t=5.384, p<0.001) . This shows that the positive psychological quality training camp can effectively enhance the quality of gratitude and self-control, and enhance the subjective well-being of higher vocational college students.