Facial temperature, due to its proximity to the brain and the abundance of blood vessels, allows it to respond more sensitively to changes in human brain activity. The temperature change of the face under the lying state was studied by setting up a simulated theft situation and preparing a special scenario to bring in the text, and the temperature change data of the three regions of the subject's face, eyes, forehead and mouth were obtained in the experimental process. After data analysis with the help of SPSS, the law of facial temperature change under the lying state was summarized, and it was concluded that the temperature change in the three regions was the most obvious in the eye, followed by the mouth, and again in the frontal area. Through the calculation and analysis of the data, it is found that there is a significant difference between the temperature change value of the three regions of the suspect group and the temperature change value of the three regions of the third person group. At the same time, although there is no statistically significant difference between the temperature change value of the three regions of the suspect group and the temperature change value of the three regions of the recruitment group, there are still obvious differences from the data, so the suspects, witnesses and unrelated third parties can be identified by this. The above rules can be used for in vitro lie identification, which has certain reference value for the study of in vitro polygraph.