Objective: To explore the effecacy of online naikan therapy for medical staff in medical quarantine site during the COVID-19,especially the impact on their anxiety and depression, sleep quality and well-being. Method: A total of 58 medical personnel who worked at the No. 25 medical quarantine site in Pudong New Area, Shanghai from January 2022 to December 2022 were randomly divided into a study group and a control group. The study group conducted a 7-day online naikan therapy. Both groups were evaluated using Self-rating Anxiety Scale (SAS), Self-rating Depression Scale (SDS), Pittsburgh Sleep Quality Index (PSQI), and Global Well-being Scale (GWB) before and after enrollment, as well as at 4, 8 and 12 weeks after treatment. Results: The SAS score of the research group was significantly lower than that of the control group at the time of after treatment and 4W (t=-5.459, -2.817, p<0.01); After treatment, the total PSQI score and sleep duration factor score of the study group were significantly lower than those of the control group (t=-2.625, -2.949, p<0.05); The GWB score of the research group was significantly higher than that of the control group at all time points (t=3.295, 4.254, 4.432, 4.496, p<0.01). Conclusion: Online Naikan therapy has a good therapeutic effect on alleviating emotional disorders, improve their sleep quality and enhance their sense of happiness among medical personnel in medical quarantine site.