With the advent of the new employer economy centered around employees, the human resource management practices of organizations are gradually shifting from “filling gaps” to “emphasizing advantages”. Strengths-based psychological climate can have a positive impact on the attitude and behavior of employees. Taking charge behavior, as a key factor in improving organizational performance, has received widespread attention from both academia and practice. Therefore, based on the proactive motivation model, this study used multiple linear regression to test the collected 351 valid questionnaires, to explore the impact of strengths-based psychological climate on employee taking charge behavior, as well as the mediating effect of job competence and job crafting and the moderating effect of task difficulty. The results indicate that: (1) Strengths-based psychological climate positively affects employees’ taking charge behavior; (2) Both job competence and job crafting partially mediate the relationship between strengths-based psychological climate and taking charge behavior; (3) Task difficulty has a positive moderating effect on the impact of strengths-based psychological climate on job competence and job crafting. This study enriches existing theoretical research to a certain extent and has certain reference for management practice.