Undergraduate students in physical education colleges of normal universities are responsiblefor the mission of physical education, and the cultivation of their physical literacy has a decisive influenceon this outcome. Coupled with the widespread application of online education, this study chose physicaleducation undergraduates from normal universities as the research object; it focused on quantitativeresearch and uses SPSS 26 as the analysis method to explore the self-efficacy of online courses and crossfieldlearning. The research results showed that there was no significant difference in the analysis ofdifferences in cross-domain learning efficacy between grades or online courses, indicating that even ifstudents were familiar with the operation mode of online courses, but there was still space to improve intheir ability of learn independently or think flexibly.