Well-being is an important indicator of individual mental health. Intentional self-regulationcan positively predict the level of well-being. In order to explore the relationship between intentionalself-regulation and well-being and their grade differences, the Intentional Self-regulation Scale and theOxford Well-being Questionnaire were applied in this study. 674 questionnaires were distributed tocollege students in five universities of Guangdong Province, and 609 valid questionnaires were recovered,with an effective rate of 90.35%. There are 188 male participants, accounting for 30.8%, and 421 femaleparticipants, accounting for 69.2%. There are 193 freshmen, accounting for 31.7%, 217 sophomores,accounting for 35.6%, 113 juniors, accounting for 18.6%, and 86 seniors, accounting for 14.1%. SPSS25.0was employed to analyze the data and results show that: (1) Intentional self-regulation is significantlyrelated to well-being; (2) In terms of well-being, there is no significant difference in grades, genders,majors of arts and sciences, and being an only child, which reflects stable characteristics of well-being;(3) With regard to intentional self-regulation, there are significant differences in grades, but there are nosignificant differences in genders, majors of arts and sciences, and being an only child, which also displaysthe rising and then falling trend of intentional self-regulation.