Objective: Discuss the situation of non-suicidal self-injury (NSSI) and its influencing factorsof private higher vocational college, and provide reference for mental health education of private highervocational colleges. Methods: 1055 students from a private higher vocational college in Zhejiang Provincewere investigated by using so jump online questionnaires. Binary Logistic regression analysis modelwas used to analyze the risk factors of NSSI behavior in private vocational college students. Results: Thedetection rate of NSSI behavior in private higher vocational colleges was 30.24% (319/1055). Univariateanalysis showed that students with family members or personal history of mental illness had a higherincidence of NSSI. There are significant differences in NSSI behavior among students with intimate andharmonious family atmosphere, intimate and alienated, intimate and tense, harmonious and alienated,harmonious and tense students. Binary Logistic regression analysis showed that family members with ahistory of mental illness, personal history of mental illness, high degree of Internet addiction, low paternalintimacy, low maternal intimacy, and depression were risk factors for NSSI. Conclusion: Family memberswith a history of mental illness, a personal history of mental illness, a high degree of Internet addiction,low paternal intimacy, low maternal intimacy, and manifestations of depression are risk factors for NSSI.