Objective: To understand the sources of psychological stress and related factors of nursinginterns (nursing students), analyze the causes, and provide relevant basis for nursing teaching andclinical teaching. Methods: Semi-structured in-depth interviews and questionnaire research were used toconduct in-depth research on the sources of psychological stress and related factors of 80 nursing internsin 2 third-class hospitals in Shanghai. Colaizzi profiling data analysis method was used to qualitativelyanalyze, summarize and summarize the collected interview data and questionnaire data. Conclusion:The psychological stress of nursing students during the internship period mainly comes from the risk ofoverloaded work and occupation. pressure on self-worth; The development of nursing disciplines andthe lag in education, etc. The above pressures seriously affect the working status of nursing interns. It notonly affects the learning efficiency, but also affects the clinical internship of nursing students, resulting innursing students being afraid to do things in the internship operation, unable to grasp some importantlearning opportunities during the internship period, and not confident in the future. Moderate stress has apositive effect on the mood and motivation of nursing students during internships, but once occupationalstressors exceed their capacity, they will damage their physical and mental health. Discussion: How toidentify and effectively resolve the psychological stress of nursing interns in a timely manner? How tointegrate the psychological literacy of nursing profession into the existing nursing education and trainingsystem and improve the comprehensive psychological literacy of nursing interns? How can nursinginterns be helped to face positive professional bias and build career confidence? Results: A 360-degreeinternship nursing psychological support system was established to regularly maintain the mental healthof nursing students, and when there was psychological pressure that was difficult to adjust, professionalpsychological help could be sought in time to eliminate and resolve stressors as soon as possible. Carryout training on psychological literacy of nursing interns to improve professional psychological quality;Regularly organize nursing occupational mental health publicity: set an example to help build professionalconfidence, clarify career goals and plans, help develop professional ethics of dedication and high sense ofresponsibility, and guide intern nursing students to correctly face professional prejudice and psychologicalpressure.