To explore the effect of English learning burnout on learning motivation of medical students, and the mediating role of learning strategy between learning burnout and learning motivation. Medical students were taken as research objects, and the Foreign Language Learning Burnout Scale, the Foreign Language Learning Motivation System Measuring Tool and the English Learning Strategy Questionnaire were used to conduct a questionnaire survey on medical students, and a total of 219 valid questionnaires were collected. The results show that: (1) English learning burnout of medical students is significantly negatively correlated with learning motivation and learning strategy, while learning motivation and learning strategy are significantly positively correlated. (2) English learning strategy partially mediate the relationship between learning burnout and learning motivation. In conclusion, the level of English learning burnout has a negative effect on their learning motivation, and part of this effect is through learning strategy.