Objective Explore fatigue and positive/negative effects and discuss countermeasures of Medical staff in the fever clinic of military hospital during the post-epidemic period. Methods The convenience sampling method was used to investigate 132 medical staff in the fever clinic of three military hospitals from January to February 2021 by using general demographic data questionnaire, fatigue scale-14 (FS-14) and positive/negative effect scale (PANAS). Results The scores of physical fatigue, mental fatigue and total fatigue were (3.612.50)、(1.581.60)and(5.183.42), respectively. The scores of positive effect and negative effect were(27.616.17)and(16.254.37), respectively. There was a significant negative correlation between positive effect and fatigue scores (P<0.01), and there was a significant positive correlation between negative effect and fatigue scores (P<0.05). Positive and negative effects significantly affected the fatigue degree (F=23.127, P <0.001). Conclusion Certain physical and mental fatigue were shown in the medical staff of the fever clinic of military hospital. However,the positive and negative effects of medical staff can directly affect their fatigue degree. Professional psychological emergency response teams for public health emergencies should be established,a psychological crisis intervention system for medical staff should be constructed,spiritual and material support should be provided to help improve their physical and mental well-being and their ability to cope with stress.