Based on previous studies, we further explore the structure of Chinese neuroticism, aiming to develop a comprehensive and accurate measurement to reflect Chinese neuroticism. Using literature review and expert consultation to develop initial scale, via two rounds of testing, we obtained Chinese neuroticism trait scale (CNTS), which totally consists of 20 items, two subscales-positive emotionality (positive and optimistic) and negative emotionality (depressed, anxious, impulsive and emotional). Then, a large sample size was collected to establish national norm. The results showed that the scale accounted for 59.349% of the total variance with good reliability and validity. The norm showed that Chinese neuroticism ranged 59.07±7.287 (positive emotionality ranged 21.61±6.944, while negative emotionality ranged 31.46±11.970). There was no difference in the total score of neuroticism in gender, age and education level, but some differences in two subscales in age and educational background. Specifically, the younger the participants were,the higher positive emotionality,whilst the lower negative emotionality they reached. Moreover,the lower educational background they got,the lower positive emotionality and the higher negative emotionality they reached. In conclusion, CNTS is applicable to Chinese to reflect their neuroticism.