Objective: To explore the mediating role of emotion regulation strategies in learning motivation and input. Methods: Zhou Bucheng's learning motivation diagnostic test (MAAT) scale was used to find out whether middle school students had behavior problems in learning motivation, interest and goal setting; Shaofley's (2002) study input scale (UWES-S) was used to study students' vitality, dedication and concentration. A total of 729 students in Hengshui, Beijing and Shijiazhuang were selected for questionnaire survey and data analysis. Conclusion: emotional regulation plays an intermediary role in the learning motivation and learning input of middle school students, accounting for 28.11% of the total effect. Emotional regulation strategies enable students to formulate their own learning motivation and greatly improve their learning input. The students' desire in the four dimensions of learning motivation is more appropriate, the difference of learning motivation in the dimension of excessive motivation is not obvious, and the difference of male and female survival in the other three dimensions is significant. There were significant differences in expression inhibition of emotion regulation strategies between male and female students. Each dimension of learning motivation has a significant negative correlation with each dimension of emotion regulation and learning input, and each dimension of emotion regulation strategy has a significant positive correlation with each dimension of learning input.