Preschool education, as the starting stage of lifelong education, plays a crucial role in individual growth and social development. This study focuses on the role of school enterprise cooperation in enhancing the practical employment abilities of preschool education majors. In depth analysis of the era significance of enhancing the employment practice ability of preschool education majors under the background of the new era, sorting out the current difficulties faced in the training process, and proposing a series of targeted practical approaches, aiming to provide theoretical and practical guidance for cultivating high-quality preschool education professionals through school enterprise cooperation, and promote the high-quality development of preschool education.