This article focuses on the construction and reform of the innovation and entrepreneurship education curriculum system in application-oriented undergraduate universities. By analyzing the existing problems in the current curriculum system, including vague positioning of curriculum objectives, disconnection between curriculum content and practice, and single teaching methods, it puts forward a series of reform paths such as clarifying curriculum objectives, constructing integrated curriculum content, innovating teaching methods, strengthening the construction of teaching staff, and improving the curriculum evaluation system. The aim is to enhance the quality and effectiveness of innovation and entrepreneurship education in application-oriented undergraduate universities and cultivate innovative and application-oriented talents who can meet the needs of society.