Since online learning has entered the public’s view, how to improve the learning effect in this scenario has always been of great concern to the public. At the same time, retrieval practice has gradually entered the educational field in recent years and has significant advantages in various aspects compared with classical strategies such as elaborative strategy. In view of this, this paper systematically analyzes the impact of retrieval practice on the online learning effect, explores its mechanism from the aspects of cognitive load theory and contextual assumption theory, and finally, on this basis, proposes teaching application methods such as using pre-class tests reasonably in the teaching process, guiding students to increase the frequency of classroom mental input activities, and scientifically designing the types of questions and practice periods that are aimed at promoting the learning effect of students in online learning contexts. The aim is to promote the improvement of students’ learning effects in the online learning context.