Few scholars pay attention to the research on the after-school service of physical education for college students. By using the methods of questionnaire and interview, this paper analyzes the evaluation of after-class service of physical education carried out by students of different genders and categories based on the present situation of the rationality, security degree and effect of after-class service of physical education in Tongji University. The results show as follows: (1) students who participate in PE after-class service participate in 1-2 times a week, and most of them are arranged in the afternoon. (2) the overall evaluation of the rationality of the after-class service of physical education is high and there is no gender difference, but there is a very significant difference between undergraduates and graduate students. (3) the overall evaluation of the degree of security of the after-class service of physical education in the school is high, and there is no gender difference, and there is no difference in student categories. (4) the overall evaluation of the effect of students’ participation in the after-class service of physical education is high and there is no gender difference, but there is a significant category difference between undergraduates and postgraduates. (5) students have a wide demand for extracurricular sports services and have more room for service expansion. And the corresponding suggestions are put forward according to the results.