The allocation of burden of proof for medical negligence is an intersection of medical issues and litigation procedural issues. In order to balance the unequal status of both parties in litigation, reduce the burden of proof on patients, and alleviate the potential litigation risks they may face, the legislative burden of proof for medical negligence has undergone a transformation from "upright" to "inverted" and then to "upright". Referring to the proof of medical negligence in foreign laws, it is also measured in favor of the patient, and different principles have been applied in theory and precedent to reduce the standard of proof for negligence, which has certain reference value for the proof of medical negligence in China. Currently, the fault presumption rule is used as the principle of liability for medical malpractice, but there are still certain shortcomings in addressing the three types of presumed fault situations. Judicial interpretations should make targeted refinement of legal norms and combine the theory of obstruction of proof to give judges sufficient discretion in determining medical behavior, so as to flexibly respond to the diverse medical malpractice behaviors that occur in practice and resolve the dilemma of proving medical malpractice.