The 31st Universiade will be held in Chengdu on July 28, 2023. During the preparation for theUniversiade, the construction and transformation of stadiums and gymnasiums, the creation of sportsatmosphere and the spread of sports spirit will all affect the sports cultural environment of collegesand universities. Through the three dimensions of sports spiritual and cultural environment, physicalenvironment of sports culture, and cultural environment of sports system, this paper studies the situationof sports cultural environment of colleges and universities in Chengdu before and after the preparationof the Universiade, so as to understand the impact of the Universiade on the sports cultural environmentof colleges and universities in Chengdu. The research shows that: (1) The Universiade has obviouslypromoted the sports spiritual and cultural environment of Chengdu universities. (2) The Universiade haseffectively promoted the physical environment of sports culture in Chengdu’s colleges and universities. (3) Inthe study of the cultural environment of the sports system, we have not found that the preparation of theUniversiade has a significant impact on it.