Modern instrumental analysis technology is a basic skill that must be mastered by undergraduate students majoring in chemistry and chemical engineering, and the experimental teaching of instrumental analysis is very important to the improvement of students' professional skills and the cultivation of engineering practice ability. According to the characteristics of chemistry and chemical engineering specialty, this paper summarizes the problems existing in the practical teaching of instrumental analysis in chemical and chemical engineering specialty, and explores and reforms the practical teaching content, teaching methods, teaching means and assessment methods. Practice has proved that the reform has greatly improved the students' learning enthusiasm and initiative, the practical innovation ability has been significantly enhanced, and the practice teaching effect has been more significant.