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Modern Analytical Chemistry Research

ISSN Print:2707-4765
ISSN Online:2707-4773
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HPLC 法测定小儿氨酚烷胺颗粒中对乙酰氨基酚和咖啡因含量

HPLC method was used to determine the content of acetaminophen and caffeine in infantile aminophenolamine granules

Modern Analytical Chemistry Research / 2019,1(2):50-55 / 2019-12-27 look787 look879
  • Authors: 毛恩平     
  • Information:
    河南中医药大学,郑州
  • Keywords: 小儿氨酚烷胺颗粒;对乙酰氨基酚:咖啡因:高相液相色谱法:含量 测定
  • Infantile paracetamol amine granules; Acetaminophen: caffeine: high phase liquid chromatography: determination of content
  • Abstract: 目的:建立测定小儿氨酚烷胺颗粒中对乙酰氨基酚和咖啡因含量的高 效液相色谱(HPLC)法。方法:用十八烷基硅烷键合硅胶为填充剂,以用三 乙胺调pH 至3.0 的0.025mol/L 磷酸溶液- 乙腈(85:15)为流动相,检测波 长为271mm,进样量为20μL。结果:对乙酰氨基盼和咖啡因的线性范围分别 为120 ~ 2000μg/mL(r=0.9999)和2.4 ~ 200μg/mL(r=0.9999)。对乙酰氨 基酚的平均回收率为100.33%,RSD=0.56%(n=5);咖啡因的平均回收率为 102.59%,RSD=l.75%(n=5)。结论:该法简单、快速、准确、稳定、重现性好。
  • Objective: to establish a high performance liquid chromatography (HPLC) method for the determination of acetaminophen and caffeine in infantile aminophenolamine granules. Methods: using octadecylsilane bonded silica gel as filler, 0.025mol/L phosphoric acid-acetonitrile (85:15), whose pH was adjusted to 3.0 by triethylamine, was used as mobile phase, the detection wavelength was 271mm, and the injection volume was 20μL. Results: the linear ranges of paracetamopion and caffeine were 120~2000 g/mL (r=0.9999) and 2.4~200 g/mL (r=0.9999), respectively. The mean recovery rate of acetaminophen was 100.33%, RSD=0.56% (n=5). The average recovery of caffeine was 102.59%, RSD=l.75% (n=5). Conclusion: the method is simple, fast, accurate, stable and reproducible.
  • DOI: https://doi.org/10.35534/macr.0102008c
  • Cite: 毛恩平.HPLC 法测定小儿氨酚烷胺颗粒中对乙酰氨基酚和咖啡因含量[J].现代分析化学研究,2019,1(2):50-55.
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