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HPLC 法测定小儿氨酚烷胺颗粒中对乙酰氨基酚和咖啡因含量

HPLC method was used to determine the content of acetaminophen and caffeine in infantile aminophenolamine granules

现代分析化学研究 / 2019,1(2)：50-55 / 2019-12-27 672 784

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• 作者： 毛恩平     
• 单位：

  河南中医药大学，郑州

• 关键词： 小儿氨酚烷胺颗粒；对乙酰氨基酚：咖啡因：高相液相色谱法：含量 测定
• Infantile paracetamol amine granules; Acetaminophen: caffeine: high phase liquid chromatography: determination of content
• 摘要： 目的：建立测定小儿氨酚烷胺颗粒中对乙酰氨基酚和咖啡因含量的高 效液相色谱（HPLC）法。方法：用十八烷基硅烷键合硅胶为填充剂，以用三 乙胺调pH 至3.0 的0.025mol/L 磷酸溶液- 乙腈（85：15）为流动相，检测波 长为271mm，进样量为20μL。结果：对乙酰氨基盼和咖啡因的线性范围分别 为120 ～ 2000μg/mL（r=0.9999）和2.4 ～ 200μg/mL（r=0.9999）。对乙酰氨 基酚的平均回收率为100.33%，RSD=0.56%（n=5）；咖啡因的平均回收率为 102.59%，RSD=l.75%（n=5）。结论：该法简单、快速、准确、稳定、重现性好。
• Objective: to establish a high performance liquid chromatography (HPLC) method for the determination of acetaminophen and caffeine in infantile aminophenolamine granules. Methods: using octadecylsilane bonded silica gel as filler, 0.025mol/L phosphoric acid-acetonitrile (85:15), whose pH was adjusted to 3.0 by triethylamine, was used as mobile phase, the detection wavelength was 271mm, and the injection volume was 20μL. Results: the linear ranges of paracetamopion and caffeine were 120~2000 g/mL (r=0.9999) and 2.4~200 g/mL (r=0.9999), respectively. The mean recovery rate of acetaminophen was 100.33%, RSD=0.56% (n=5). The average recovery of caffeine was 102.59%, RSD=l.75% (n=5). Conclusion: the method is simple, fast, accurate, stable and reproducible.
• DOI： https://doi.org/10.35534/macr.0102008c
• 引用： 毛恩平．HPLC 法测定小儿氨酚烷胺颗粒中对乙酰氨基酚和咖啡因含量［J］．现代分析化学研究，2019，1（2）：50-55．