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利用液相色谱质谱法用于植物性蛋白中三聚氰胺的测定

Determination of melamine in plant protein by liquid chromatography-mass spectrometry

现代分析化学研究 / 2020,2(2)：41-48 / 2020-05-27 1953 3248

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• 作者： 祝险峰     
• 单位：

  河南工业大学，郑州

• 关键词： 液相色谱质谱法；植物性蛋白；三聚氰胺
• Liquid chromatography-mass spectrometry; plant protein; melamine
• 摘要： 建立三重四极杆液相色谱质谱联用分析植物性蛋白中三聚氰胺的检测 方法。方法：样品用 1% 乙酸 + 乙腈（4 ∶ 1，V/V）提取，固相萃取柱净化， 利用液质联用的多反应监测（MRM）技术，对三聚氰胺进行定性定量分析，以 三聚氰胺的离子对 m/z127/65 进行定性，以离子对 m/z127/85 进行定量。结果： 方法在 0.025 μg/mL ～ 0.400 μg/mL 的范围内呈良好的线性关系，加标平均回 收率为 87.2% ～ 99.9%，试验的 RSD 为 0.51% ～ 1.23%。结论：该方法通过优化 仪器分析条件，操作方便，重现性好，回收率高，结果准确，为三聚氰胺的检 测提供了准确可靠的方法。
• An inspection method of triple quadruple liquid chromatography-mass spectrometry was developed for the determination of melamine in plant protein. Methods: samples were extracted with 1% acetic acid + acetonitrile (4:1, V / V), and purified through a solid phase extraction column. Qualitative and quantitative analysis of melamine was performed by using the multiple reaction monitoring (MRM)technology of liquid chromatography-mass spectrometry, and the ion pairs m/ z127> 65 and m/z127> 85 of melamine were used for qualification and quantification respectively. Results: the developed method presented good linearity in the range of 0.025 μg/mL to 0.400 μg/mL. The average recoveries were 87.2 % to 99.9 %, and the RSD was 0.51 % to 1.23 %. Conclusion: by using the optimal instruments, the method has the advantages of easy operation, good reproducibility, high recovery rate and accurate results for the detection of melamine.
• DOI： https://doi.org/10.35534/macr.0202007c
• 引用： 祝险峰．利用液相色谱质谱法用于植物性蛋白中三聚氰胺的测定［J］．现代分析化学研究，2020，2（2）：41-48．