Objective To investigate the asymmetric characteristics of binaural hearing ability of shooting athletes, andto explore the relationship between hearing loss caused by gun noise exposure and binaural asymmetry.Methods Across-sectional survey method was adopted to detect binaural air conduction pure tone (0.128, 0.256, 0.512, 1, 2, 3,4, 6, 8 kHz) of first-line shooters in Hubei Province from January 2023 to July 2023 with a type BD-II-116 auditoryexperimental instrument, and a questionnaire survey was conducted. The auditory thresholds of left and right earsat different frequencies were compared by repeated measurement analysis of variance (ANOVA). The difference ofhearing threshold in each frequency band was statistically analyzed by paired sample t test. Results Among the 9frequency bands tested, except 0.128KHz band, the hearing threshold of the shooters’ left ear is higher than that of theright ear in all other frequency bands. In the frequency bands of 0.512 kHz, 1 kHz, 2 kHz, 3 kHz, 4 kHz, 6 kHz and 8 kHz, the difference of hearing threshold between left and right ears was statistically significant (P<0.05 or P<0.01),and the average hearing threshold of left ear was 2.04dB higher than that of right ear. The difference of binauralhearing threshold is the largest in 8KHz band, which is (4.95±4.15) dB. With the increase of frequency, the numberof shooters with binaural hearing threshold asymmetry is also increasing, and the dispersion of hearing thresholddifference is also greater. Among the athletes with large difference of binaural threshold displacement value at highfrequency, the majority of athletes with unimpaired hearing accounted for. Conclusion The problem of asymmetricauditory ability of shooting athletes has begun to appear, the average auditory threshold of left ear is higher thanthat of right ear, which mainly occurs at high frequency. With the increase of frequency, the number of asymmetryof binaural hearing ability increased, and the asymmetry of binaural ability became more serious. Although someathletes do not have hearing loss, the asymmetry of binaural hearing ability is still more prominent.