To investigate the differences in functional performance movement screening between gymnastics-specialized and non-specialized students, and to analyze the factors for the differences. Sixteen undergraduate students enrolled in a university in Wuhan were selected for the FMS scoring, respectively eight gymnastic-specialized and eight non-specialized sports majors. Among the results of the FMS tests of the gymnast-specialized and non-specialized students, there was a significant difference in the test of straight-leg active lifting; the results of the left and right sides of the FMS tests of the gymnast-specialized and non-specialized students showed that there was a significant difference between the left side and the right side of straight-leg active lifting, and there was a significant difference between the right side and the right side of the turnover stability only; there was a significant difference in the tests of the items between different genders in the body control push-ups and turnover stability; and there was a significant difference between different genders in the tests of different items. There were significant differences between the left and right sides of the FMS tests for each event between genders; there were significant differences between the left and right sides of the straight leg active lift and the turn stability. Gymnastics specialization is better than no specialization students flexibility, habitual right limb stability is better than the left side performance, leg flexibility left side but better than the right side performance; boys in the strength and body control is better than the girls, while the boys left side of the body control is worse, and, the girls left side of the leg flexibility is better.