Under the support of the Internet and other technologies, online gambling crimes have become increasingly rampant, and criminals have begun to use offshore gambling syndicates and gambling websites to carry out money laundering criminal activities. In the face of the emergence of new money laundering modes such as virtual coins, false e-commerce, and score running in network gambling crimes, the investigation of cases faces the difficulties in filing investigation and verification, financial monitoring technology identification and cross-border information sharing and collaboration, etc. This paper applies the case study method and literature research method to focus on the status quo of the money laundering cases of network gambling and the difficulties in the investigation, and provides investigation countermeasures from the aspects of case filing, technology, and collaboration with a view to maintaining the socialist market order, reduce the possibility of financial market crime, and improve the system of money laundering risk prevention and control in China.