The traditional method of manual examination of tool mark is challenged in the court for its subjectivity. With reference to the challenging, the computer-based approach have been studied in the world. The approach mainly focused on extraction of striation feature and statistical examination of striations. The machine learning method was studied, and four groups of experiments were conducted with a 2D image dataset of tool marks made by screwdrivers, cutting pliers and bolt clippers. The four LBP derivatives operators were developed to extract the tool-mark features and then construct the features into a feature vector. The random forest algorithm was adopted to identify the labeled feature vectors by supervised learning. The experimental results show that the proposed method achieved a high-rate of identification of the striated marks generated under identical conditions, and reduced the uncertainty of results examined by traditional method. Furthermore, the proposed method is immune to the unstable illumination when the image data of the striated marks are collected, and avoids the difficulty in mark inspection caused by manually preset parameters in the existing methods.