The anesthetic effect of different concentrations of MS-222 on Brachymystax lenok Pallas was observed. The results showed that the anesthetic effect of juvenile fish was a gradual process, which can be divided into four groups: non-anaesthesia, light anesthesia, moderate anesthesia and deep anesthesia. There were differences in the time of entering different anaesthesia degree at different concentration of MS-222. In the process of 900 s treated with MS-222, 100% of juvenile fish showed light anaesthesia under the concentration of 30 mg/l. At 301.5 s, juvenile fish all entered mild anesthesia during the concentration of 35 mg/l, 567 s showed moderate anesthesia. At 57.5 s, juvenile fish all entered mild anesthesia during the concentration of 40 mg/l, 638.5 s showed deep anesthesia. During the experiment, 100% of 45 mg/l ~ 55 mg/l showed deep anesthesia, and the duration of all anesthesia was shortened gradually. When the concentration exceeded 45 mg/l, and the anesthesia time exceeded 12 hours, the juvenile fish died. Therefore, attention should be paid to the concentration and time of the anesthetic MS-222.